Table 1 Alteration of total sterol composition in response to erg6 repression

From: The sterol C-24 methyltransferase encoding gene, erg6, is essential for viability of Aspergillus species

Type of sterol

ΔakuB‐pyrG + 

pTetOff-erg6

NT

0.5 µg/mL Dox

2 µg/mL Dox

NT

0.5 µg/mL Dox

2 µg/mL Dox

Ergosta-5,8,22,24(28)-tetraenol

0.4 ± 0.3

0.4 ± 0.1

0.6 ± 0.2

1.4 ± 0.4

0.3 ± 0.3

0.6 ± 0.5

Cholesta-5,7,24-trienol

    

1.4 ± 0.7

0.7 ± 0.7

Ergosta-5,8,22-trienol

0.9 ± 0.2

1.2 ± 0.6

0.8 ± 0.2

1.4 ± 0.4

  

Cholesta-dienol

    

1.1 ± 0.4

0.7 ± 0.7

Ergosterol

90.4 ± 1.3

90.2 ± 2.2

90.5 ± 1.6

88.6 ± 2.8

47.4 ± 5.5

43.4 ± 5.0

Ergosta-5,7,22,24(28)-tetraenol

    

0.3 ± 0.4

 

Cholesta-5,7,22,24-tetraenol

    

2.5 ± 0.4

3.6 ± 0.8

4,4-Dimethyl cholesta dienol

    

2.6 ± 0.1

1.9 ± 1.7

Ergosta-5,7,24(28)-trienol

1.1 ± 0.4

0.7 ± 0.5

1.1 ± 0.7

0.4 ± 0.7

  

Ergosta-5,7-dienol

1.3 ± 0.2

1.3 ± 0.2

1.0 ± 0.2

0.9 ± 0.9

  

Episterol [Ergosta-7,24(28)-dienol]

0.8 ± 0.2

0.7 ± 0.2

0.7 ± 0.1

0.8 ± 0.1

  

Lanosterol

0.6 ± 0.1

0.5 ± 0.2

0.6 ± 0.3

0.5 ± 0.5

39.8 ± 6.0

42.7 ± 4.4

4-Methyl ergosta-8,24(28)-dienol

0.8 ± 0.1

0.8 ± 0.1

0.7 ± 0.1

1.0 ± 0.2

  

Eburicol

1.0 ± 0.2

1.0 ± 0.1

1.0 ± 0.1

1.5 ± 0.5

1.3 ± 1.2

2.4 ± 2.2

4,4-Dimethyl ergosta-8,24(28)-dienol

1.4 ± 0.1

1.4 ± 0.2

1.3 ± 0.5

1.9 ± 0.9

  
  1. Data presented as means for 3 replicates with standard deviation are percentage of the total sterol composition. Values of >2% of the total sterol composition are shown in bold. NT No Treatment.
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