Fig. 4: Endoderm metacell subclustering. | Nature Communications

Fig. 4: Endoderm metacell subclustering.

From: An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm

Fig. 4

a 2D projection of endodermal metacells on a side view scheme of an amphioxus neurula stage embryo with anterior to the left and dorsal to the top. b Gene expression as normalized fold changes of selected gene markers and corresponding ISH (n = 10 embryos). Gene expression is shown as density maps representing UMI counts (per 10,000 UMIs) in each cell, with cells positioned in the vicinity of their corresponding metacells. c Multiple in situ hybridization for Dmbx, Fgfrl and Brachyury2 (n = 5 embryos). Lateral views are shown on the left, with anterior to the left and dorsal to the top. Optical cross-sections at the level of the dotted line are also shown. The labeling intensity is stronger on one side due to the thickness of the embryo that was imaged on one side after mounting. The three genes are coexpressed in the anteriormost region of the dorsal mesendoderm (metacell 1). Normalized fold change expression of each marker is shown on the right. Scale bar: 25 µm.

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