Fig. 5: Somite metacell subclustering. | Nature Communications

Fig. 5: Somite metacell subclustering.

From: An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm

Fig. 5

a 2D projection of somitic metacells on a side view scheme of an amphioxus neurula stage embryo, with anterior to the left and dorsal to the top. b Gene expression as normalized fold changes of selected gene markers and corresponding ISH (n = 10 embryos). c Multiple in situ hybridization for Ripply+Alx+Gata1/2/3 and Ripply+Tcf21/Msc+Alx (n = 5 embryos). Lateral views are shown on the left, with anterior to the left and dorsal to the top. Optical cross-sections at the level of the dotted line are also shown. The labeling intensity is stronger on one side due to the thickness of the embryo. Ripply, Alx and Gata1/2/3 are coexpressed in the non-muscular part of formed somites (metacells 2 and 11) whereas Ripply, Tcf21/Msc and Alx are coexpressed in the first somite pair (metacells 1 and 3). Scale bar: 25 µm.

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