Fig. 6: Phenotype of N4 stage embryos after overexpression of a constituve repressor form of Gata1/2/3. | Nature Communications

Fig. 6: Phenotype of N4 stage embryos after overexpression of a constituve repressor form of Gata1/2/3.

From: An amphioxus neurula stage cell atlas supports a complex scenario for the emergence of vertebrate head mesoderm

Fig. 6

Control embryos (n = 5), injected embryos with a mild phenotype (n = 5/10) and injected embryos with a strong phenotype (n = 5/10) from two independent injection experiments were processed for double in situ hybridization for MLC-alk and Alx followed by immunostaining against Laminin. Side views of 3D reconstructions of the nuclei and Laminin labeling are shown on the top, with anterior to the left and dorsal to the top. Sections in side views showing all the chanels are shown below, as well as transverse optic sections at the level indicated by the white lines. MLC-alk is expressed in the myotome region of the somites that is little affected. Alx is expressed in the ventral region of the somites that is completely disorganized in injected embryos, as revealed by anti-Laminin labeling. Scale bar: 25 µm.

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