Fig. 7: Lack of galectin-3 in mouse increases lung bacterial burden with suppression of IFN-γ-expressing neutrophils.

a Bacterial load showing increased bacterial burden in the lungs of KO mice in both PA14 and LPS + PA14 groups as compared to the respective groups in WT mice (assayed 4 h post-infection). Data were log transformed using log base 10 to adjust for differences in standard deviation prior to analysis. Data pooled from 3 independent experiments with n = 10 mice per group. Data analyzed using Brown Forsythe and Welch ANOVA test with Dunnett’s T3 comparisons. Flow cytometry analysis showing total numbers of lung cells (b), CD11b⁺ cells (c), and neutrophils (d) in both WT and galectin-3 KO LPS + PA14 mice. Total cell counts (e) and representative flow plots (f) of IFN-γ-expressing neutrophils in the lungs of both WT and KO LPS + PA14 mice. b–f Data pooled from 3 independent experiments where n = 10 mice per group. g Flow cytometry analysis showing total BAL cells in WT and KO mice. h Intracellular bacterial load in BAL cells assessed by permeabilizing BAL cells from WT and KO mice. Data pooled from 2 independent experiments with n = 3 mice per group. i Bacterial burden in lungs of LPS + PA14 infected KO mice without or with adoptive transfer of BAL neutrophils isolated from LPS treated WT mice. g–i Data pooled from 2 independent experiments with n = 7 mice per group. b–e, g–i Data analyzed using two-tailed unpaired t test with Welch’s correction. All data are presented as mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Source data and exact P values are provided as a Source Data file.