Fig. 4: OsSRF8 interacts with OsINP1.
From: OsSRF8 interacts with OsINP1 and OsDAF1 to regulate pollen aperture formation in rice
a Yeast two-hybrid (Y2H) assay verified the interaction of OsSRF8 and OsINP1. OsSRF8 (N) and OsSRF8 (C) represent the N-terminal domain (amino acids 26-320) and C-terminal domain (amino acids 344-757) of the OsSRF8 protein, respectively. AD activating domain, BD DNA-binding domain, SD synthetic defined medium. b Split-luciferase assay testing the interaction between OsSRF8 and OsINP1 in the tobacco (Nicotiana benthamiana) leaves. c Interaction between OsINP1 and OsSRF8 detected using Bimolecular fluorescence complementation (BiFC) assay. OsINP1 and OsSRF8 respectively were fused to the C-and N-terminal parts of YFP and co-transformed into tobacco leaves. Scale bars, 50 μm. d Co-immunoprecipitation experiments in the tobacco leaves. α-Flag IP indicates immunoprecipitation with anti-Flag antibody beads. All experiments were repeated three times, with similar results.
