Fig. 2: sodB responds positively to phosphorus starvation. | Nature Communications

Fig. 2: sodB responds positively to phosphorus starvation.

From: Phosphorus deficiency alleviates iron limitation in Synechocystis cyanobacteria through direct PhoB-mediated gene regulation

Fig. 2

a The intracellular ROS accumulation of Synechocystis after 4 days of growth under different iron and phosphorus availabilities. Values are mean ± SD of three independent biological replicates, and the results were normalized to the ROS level of +Fe+P treatment. b Relative mRNA abundance of sodB in Synechocystis after 4 days of growth under +Fe+P, +Fe−P, −Fe+P, and −Fe−P conditions. Data shown are mean ± SD of three independent biological replicates, and the results were normalized to the transcript levels measured under +Fe+P conditions. c SOD activity of Synechocystis determined by in-gel activity staining. Samples were loaded on an equal total protein basis. Similar results were obtained from three independent biological replicates. d The SOD activity detected from (c) was quantified with ImageJ software. Data shown are mean ± SD of three independent biological replicates and values are given relative to SOD activities of +Fe+P samples. Letters above bars indicate statistical significance (P < 0.05) calculated by one-way ANOVA across all treatments. Line segments and corresponding P-values represent statistical significance calculated by two-sided Student’s t-test. Source data are provided as a Source Data file.

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