Fig. 1: Vaha expressed in the gut concentrates in the IPCs.

a Immunostaining for GFP in the brain dissected with a portion of the gut from 5-7 day old adult flies expressing Vaha GFP in the gut (w^-; NP1 Gal4/+; UASVaha GFP/+). Inset shows 3x magnified image. n = 6 guts with brains, scale bar 200 μm. b Immunoblotting with GFP antibody of extracts from guts and CNS (brain and ventral nerve cord) from flies expressing Vaha GFP (left panel) or signal peptide deleted Vaha ∆30GFP (right panel) driven by NP1Gal4. Actin is used as loading control. n = 3, 7 guts and 7 CNS for each replicate. c Immunostaining for Vaha GFP (anti GFP, green) and ILP2HF (anti HA, red) in CNS dissected from 5-7day old adult flies expressing Vaha GFP in the gut and ILP2HF under the control of its own promoter. The genotype is w^-; NP1Gal4/+; UASVaha GFP/gd2HF. ILP2HF (gd2HF) marks IPCs. Nuclei are stained with DAPI (blue). A higher magnification image of the boxed region in the merged panel is shown in the panel to its right, scale bar 50 μm. The confocal images are projection of z stacks, n = 6 CNS, scale bar 250 μm. d Immunostaining for Vaha (anti GFP) and ILP2HF (anti HA) in CNS dissected from 5-7day old adult flies expressing Vaha ∆30GFP in the gut and ILP2HF under the control of its own promoter (w^-; NP1Gal4/+; UASVaha ∆30GFP/gd2HF). Nuclei are stained with DAPI. A higher magnification image of the boxed region is shown, scale bar 50 μm. The confocal images are projection of z stacks, n = 6 CNS, scale bar 250 μm. e Immunostaining for Vaha V5 (anti V5, green) and ILP2HF (anti HA, red) in CNS isolated from 5-7day old adult flies expressing Vaha V5 under the control of its own promoter and ILP2HF (w^-; Vaha V5/VahaV5; gd2HF/gd2HF). Nuclei are stained with DAPI (blue). A higher magnification image of the boxed region is shown, scale bar 50 μm. Confocal images are projection of z stacks, n = 6 CNS, scale bar 250 μm. Source data for 1b are provided as a source data file.