Fig. 6: Vaha influences glucose metabolism through ILP.

a Immunostaining of brains dissected from 5-7 day control, vaha and vaha expressing bacterial sodium channel via dILP2Gal4 driver for ILP2HF (red) and DAPI (blue). The top panel shows brightfield images. Confocal images are projection of z stacks n = 6 brains, scale bar 100 μm. b Quantification of ILP2HF fluorescence intensity in the IPCs of flies described in a. Each data point represents fluorescent intensity in one IPC, n = 41(control), n = 35 (vaha mutant), n = 35 (vaha.dILP2Gal4>NaChBac). One-way ANOVA followed by Tukey’s multiple comparison test is used and horizontal line indicates median. c Glucose content and d TAG content measured in flies described in a. Each data point represents value from 10 flies, n = 4, One-way ANOVA followed by Tukey’s multiple comparison test is used and data is presented as mean ± SD. e Glucose content and f TAG content measured in vaha mutant, insulin mutant and combined vaha and insulin mutant flies. The genotypes of flies are control w¹¹¹⁸; vaha mutants w^-; vaha/vaha; insulin mutants w^-; +/+; ilp2,3,5/ilp2,3 and combined mutants is w^-; vaha/vaha; ilp2,3,5/ilp2,3. Each dot represents value from 10 flies, n = 4, One-way ANOVA followed by Tukey’s multiple comparison test is used and data is presented as mean ± SD. g Eclosion of adult flies described in a and e-f is followed between 216 to 312 hours after egg laying. h Glucose tolerance test performed on adult flies described in e-f. Flies fasted overnight are fed on glucose containing media for 1 h and then re-fasted for 1 h. Circulatory glucose is measured, and each data point represents value from 40 flies. n = 3, One-way ANOVA followed by Tukey’s multiple comparison test is used and data is presented as mean ± SD. Source data are provided for 6b, c, d, e, f, g, h as a source data file.