Fig. 7: vaha mutant flies display features of diabetes.

a Glucose levels were estimated in whole fly homogenates of 30–35 day old control and vaha mutant flies by UPLC-MS/MS. Data is obtained from three biological replicates with100 flies per replicate. Data is assessed by two-tailed Student’s t-test and presented as mean ± SD. b Triglyceride (TAG) levels were estimated in whole fly homogenates of 30−35 day old control and vaha mutant flies by UPLC-MS/MS. Data is obtained from three biological replicates with100 flies per replicate. Data is assessed by two-tailed Student’s t-test and presented as mean ± SD. c Heat map showing lipids with significantly altered abundance (log₂ (fold change) ≥ 0.59 and adjusted p value ≤ 0.05) in whole fly samples of the control compared to those of the vaha mutants. Columns represent samples and rows represent lipid species. The color key indicates Z-scores of the relative concentrations of lipid species with blue being the lowest and red the highest. Data is obtained from three biological replicates with 100 flies (30–35 day old) per replicate. d Heat map showing metabolites with significantly altered abundance ((log₂ (fold change) ≥ 0.59 and adjusted p value ≤ 0.05) in whole fly samples of the control compared to those of the vaha mutants. Columns represent samples and rows represent metabolites. The color key indicates Z-scores of the relative concentrations of metabolites with blue being the lowest and red the highest. Data is obtained from three biological replicates with 100 flies (30–35 day old) per replicate. Source data are provided for 7a, b as a source data file.