Fig. 7: Decreased ZO-1 expression and increased YB-1-positive granules in ECs at the vascular front of developing retinal blood vessels. | Nature Communications

Fig. 7: Decreased ZO-1 expression and increased YB-1-positive granules in ECs at the vascular front of developing retinal blood vessels.

From: ZO-1 interacts with YB-1 in endothelial cells to regulate stress granule formation during angiogenesis

Fig. 7

a Decreased ZO-1 levels in ECs at the vascular front of the developing retinal vasculature of mice. Immunofluorescence analysis of ZO-1 levels in ECs of the vascular front (edge) and plexus (central) portions of the developing retinal vasculature (P7) of mice. Vascular ECs of the retinas were stained with isolectin B4 (red) and with antibodies against ZO-1 (green). For clarity, right panels show the ZO-1 staining in black and white with intensities inverted. The histogram shows the quantification of images shown in (a). The results are presented as a ratio of the ZO-1/isolectin B4 intensity measured in n = 8 retinas (plexus or vascular front) from four mice. b, Increased YB-1-positive granules in ECs of the vascular front portion of the developing retinal vasculature. YB-1-positive granules were stained by immunofluorescence in ECs at the vascular front and in the plexus portion of the developing retinal vasculature (P7) of mice (green). ECs of the retinas were stained with isolectin B4 (red). The histogram presents the quantification of YB-1-positive granules in retinal ECs shown in (b). The percentage of isolectin B4-stained cells with YB-1 granules was calculated at the vascular front and in the plexus of the retinal vasculature (n = 4 retinas; one retina per mouse was used). c Analysis of Single-cell RNA-sequencing data of ECs from mouse retinas at P6. Uniform Manifold Approximation and Projection (UMAP) plot of EC subclusters (Tip ECs, Arterial ECs, Capillaries, Proliferative ECs, Venous ECs) from P6 retinas generated in ref. 56. d Dot plot of expression level and frequency among EC subclusters of marker genes (Esm1, Angpt2; Tip EC markers) (Top2a, Mki67; Proliferative EC markers) (Cdh5, EC marker) and of (e) selected genes Tjp1 (ZO-1), Ybx1 (YB-1) and Cldn5. (a, b) Groups were compared by unpaired two-tailed Student’s t test. Data are presented as mean values ± SEM. Source data are provided in the Source Data file.

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