Fig. 2: Binding analysis of SecR constructs expressed in cell membranes.
From: Impact of secretin receptor homo-dimerization on natural ligand binding
Shown are the competition-binding data for membranes expressing WT SecR (left panel) and non-dimerizing mutant SecR (right panel) in the absence (black) or presence (blue) of 10 µM GppNHp. Values are expressed as percentages of saturable binding and displayed as means ± S.E.M. from three independent experiments performed in duplicate, and analyzed using the Mann–Whitney test. Values of Ki for high and low affinity sites are noted, with only the high affinity site in SecR WT significantly different in the presence and absence of GppNHp at this receptor (P = 0.011)*. The affinity of this high affinity binding site in the absence of GppNHp is also significantly different in SecR WT from that in SecR(G264A,I268A) (P = 0.0136)#. (all values shown in Supplementary Table 4).
