Fig. 3: Kinetic binding profiles of Alexa488 secretin probes in WT and mutant secretin receptors.
From: Impact of secretin receptor homo-dimerization on natural ligand binding
Shown are the kinetic receptor binding profiles of fluorescent probes using fluorescence polarization at membranes bearing WT SecR (top row) and mutant SecR (bottom row). N-terminal probe (Alexa488-Sec) has a faster off rate compared to the other probes for WT SecR, while position 13 and C-terminal probes exhibited faster off rates for mutant SecR (P = 0.0133 and 0.0321, respectively, determined using one-way ANOVA with Tukey post-test). Values are expressed as means ± S.E.M. from five independent experiments performed in triplicate. Absolute values and statistical analysis are shown in Supplementary Table 5.
