Fig. 4: Fluorescence quenching of receptor-bound probes in active and G protein-uncoupled states.
From: Impact of secretin receptor homo-dimerization on natural ligand binding
Shown are the KI Stern–Volmer collisional quenching patterns of receptor-bound probes at WT SecR (top panel) and non-dimerizing mutant SecR (bottom panel) in the absence (closed circles) or presence (open circles) of GppNHp. Values are expressed as means ± S.E.M. from 3 independent experiments, and analyzed using one-way ANOVA with Tukey post-test. Shown here are comparisons reaching: * or #: P < 0.05; ** or ##: P < 0.01. Absolute values and statistical analysis are shown in Supplementary Table 6.
