Fig. 1: NF-κB signaling is enriched in AGM-derived HSCs.
From: IκBα controls dormancy in hematopoietic stem cells via retinoic acid during embryonic development
A UMAP projection colored by scRNAseq clusters where different HSC populations are highlighted. Single-cell RNA-sequencing data derived from Zhou et al.13. EC = CD31+Cdh5+CD41-CD43-CD45-; T1 PreHSC = CD31+cKIT+CD41lowCD201highCD45-; T2 PreHSC = CD31+cKIT+CD201highCD45+; E12 FL-HSC= lin-SCA+CD201highMac1low; E14 FL-HSC = CD45+CD201CD48-CD150+; adult BM-HSC= lin-SCA1+cKIT+CD135-CD34- CD48-CD150+ (B), enrichment score analysis of indicated HSC populations for inflammatory, NF-κB pathway and NF-κB signaling molecules. Boxplot shows indicate the upper- (75%) and lower- (25%) percentile, with the median value shown as a solid black line. Statistical significance determined with a two-sided t-test. No adjustments were made for multiple comparisons. *** < 0.001, ** < 0.01, * < 0.05 (C), GSEA results comparing T1/T2 HSC cells to FL-HSC (left) or endothelial cells (EC) to T1/T2 HSC for correlation to the Hallmark “TNFA_SIGNALING_VIA_ NF-κB” gene set. The indicated p-value is a nominal p-value obtained from GSEA. D UMAP projection colored by the expression of selected genes from the NF-κB signaling pathway.
