Fig. 2: Schematic diagram of main constructs in the study.
From: Germline Cas9 promoters with improved performance for homing gene drive
A The synthetic target drive is placed inside an EGFP gene at the gRNA target site. A DsRed fluorescence marker is regulated by the 3xP3 promoter for expression in the eyes together and a P10 3′ UTR element. A single gRNA driven by U6:3 promoter targets EGFP. Cas9 is driven by different compositions of promoter/5′ UTR and 3′ UTR. B The split drive Cas9 elements all contain an EGFP fluorescent marker gene driven by the 3xP3 promoter and with an SV40 3′ UTR. Cas9 is driven by different compositions of promoter/5′ UTR and 3′ UTR.
