Fig. 3: EGFP target site drive performance.
From: Germline Cas9 promoters with improved performance for homing gene drive
The chart shows the drive performance of twelve homing drive systems targeting EGFP on chromosome 2L differing by promoter/5′ UTR and 3′ UTR regulation of Cas9, or in one case, addition of a PEST sequence inside Cas9. A Drive inheritance for males and females was measured in the progeny of drive/EGFP heterozygotes. Female germline resistance was not measured, and male germline resistance and wild-type inheritance were measured from crosses with w1118 females (some male crosses were with EGFP homozygous females, and these crosses only contribute to driving inheritance measurements). Female drive heterozygotes were always crossed with EGFP males. B The fraction of offspring lacking EGFP phenotype (or with mosaic phenotype) and inheriting the drive is labeled as “Embryo resistance+somatic” because either maternally deposited Cas9 and gRNA or somatic expression in the eye could be responsible for lack of EGFP. “Embryo resistance rate” (and the corresponding mosaic rate) is similar but reports the fraction of non-drive offspring lacking EGFP, which can only be caused by maternal deposition. The leftmost drive data is from a previous study10. Error bars represent SEM. Source data is provided in Data Set S1 in the Source Data file.
