Fig. 4: yellow target site drive performance.
From: Germline Cas9 promoters with improved performance for homing gene drive
Females heterozygous for different Cas9 alleles and for the yellow drive were crossed with w1118 males. A Their progeny were phenotyped for DsRed (drive), EGFP (Cas9), and yellow body color. The germline resistance inheritance shows the fraction of male progeny with yellow body but no drive, and wild-type and r1/functional resistance indicates the fraction of male offspring that were wild-type. B The fraction of offspring with yellow (or mosaic) phenotype inheriting the drive and also inheriting Cas9 is “Embryo resistance+somatic” because either maternally deposited Cas9/gRNA or somatic expression could cause the yellow phenotype. “Embryo resistance rate” (mosaic rate) reports the fraction of drive offspring lacking Cas9 that have the yellow phenotype, which must be from maternal deposition. “Reverse” indicates a change in orientation on one gene of the allele so that the Cas9 and 3xP3 promoters are not adjacent. One Cas9 allele had different performances between lines, displayed as “line 1” and “line 2”. Error bars represent SEM. Source data is provided in Data Set S2.
