Fig. 3: Example network configuration based on cortical organoids.
A Two structures of cortical organoids modeled using FS and RS neurons connected with excitatory and inhibitory synaptic connection (AMPAR and GABAAR) based on biological culture observations and their spiking activity. Synaptic connections are promoted according to rules depending on the structure to reproduce, spatial placement of neurons and the ratio of inhibition/excitation connection observed. The network configuration was exported from Biœmus software. B Heatmap of the number of connection per neuron for the two structures of organoid interconnection showing internal and external synaptic connections. Internal connections correspond to the synaptic connections inside the two organoids of both structures in a closer neighbor connection manner. External connections correspond to synaptic connections between organoids for the connected structure, favoring synaptic connection on the exterior ring of the organoid. Heatmap are based on an average of 40 random generations of synapses for a given XY mapping. C Raster plot of 10-second emulated activity of a single and connected structure using BiœmuS, highlighting network burst synchronicity for the connected structure. The spiking activity emulated corresponds to a maximum probability for connection inside and outside the organoids of respectively 10% and 2% with 512 neurons per organoid and a 20% inhibition/excitatory neuron ratio.
