Fig. 5: Acute depletion of BRG1 results in significantly decreased p300 binding and H3K27 acetylation at BRG1 binding sites in mouse T cells.
From: Acute depletion of BRG1 reveals its primary function as an activator of transcription
a Genome browser snapshot displays H3K27ac and P300 ChIC-seq profiles, with blue tracks for wild type and red for BRG1-depleted cells. b MA plot depicts H3K27ac changes at 21,347 H3K27ac peaks defined in WT cells following 10-h BRG1 depletion in T cells, with blue and red dots marking significantly increased and decreased peaks, respectively, identified using a two-sided ROTS test (P < 0.05, fold-change> 1.3). c MA plot reveals changes in P300 binding at 39,315 P300 peaks defined in WT cells due to 10-h BRG1 depletion in T cells, with blue and red dots indicating significant increases and decreases, respectively, determined by a two-sided ROTS test (P < 0.05, fold-change> 1.3). d Violin plots compare H3K27ac levels across BRG1 binding groups (low, medium, high) in wild-type T cells at nonTSS (> 5 kb from TSS) and TSS (≤ 2 kb) regions. Only peaks overlapping with BRG1 were assessed, categorized by BRG1 levels, with P values determined using a two-sided Wilcoxon rank sum test. e Violin plots illustrate H3K27ac alterations at nonTSS (left) and TSS (right) peaks in T cells following 10-h BRG1 depletion. Peaks were grouped by BRG1 binding changes (more decrease, medium, less decrease) compared between BRG1-depleted and wild-type cells, with P values calculated by a two-sided Wilcoxon rank sum test. f Violin plots depict variations in p300 binding at nonTSS (> 5 kb from TSS) and TSS (≤ 2 kb) regions among low, medium, and high BRG1 binding groups in wild-type T cells. The analysis included only p300 peaks overlapping BRG1, categorized by BRG1 binding intensity, with P values determined by a two-sided Wilcoxon rank sum test. g Violin plots compare p300 alterations at non-TSS (left) and TSS (right) peaks in T cells following 10-h BRG1 depletion, with peaks grouped by the extent of binding decrease (more, medium, less) in BRG1-depleted versus wild-type cells. P values were calculated using a two-sided Wilcoxon rank sum test.
