Fig. 6: Acute depletion of BRG1 results in decreased chromatin accessibility and nascent transcription in hepatocytes and fibroblasts.
From: Acute depletion of BRG1 reveals its primary function as an activator of transcription
a, b Volcano plots display DNase-seq density changes at 49,891 DHSs in hepatocytes following acute BRG1 depletion (a) or conditional Brg1 gene deletion (b), with blue and red dots indicating significant increases and decreases, respectively, identified using a two-sided ROTS test (P < 0.05, fold-change> 1.3). c Volcano plot depicts changes in 81,017 BRG1 ChIC-seq binding sites in fibroblasts after 10-h auxin-induced depletion. Blue and red dots mark significantly increased and decreased BRG1 peaks, respectively, assessed by a two-sided ROTS test. d Volcano plot reveals DNase-seq read density changes at 80,732 DHSs in fibroblasts after acute BRG1 depletion, with blue and red dots showing significant increases and decreases, respectively, determined by a two-sided ROTS test (P < 0.05, fold-change> 1.3). e, f, MA plots display PRO-seq-derived changes in hepatocytes (e) and fibroblasts (f) after 10-h BRG1 depletion. Blue and red dots mark genes with significant increases and decreases, respectively, with dotted lines for no change. Significance was set at P < 0.05 and fold-change > 1.3, analyzed by a two-sided ROTS test.
