Fig. 1: SPT6L co-occupies and interacts with NRPE1 in Arabidopsis.

a Genome tracks display SPT6L, Pol II, and NRPE1 ChIP-seq signals on chromosome 1 (Chr1: 6700 to 6780 kb). The ChIP signal of each sample was averaged over two biological replicates. The y-axis values indicate the mean of normalized reads per 10 bp. The black arrows pointed to co-binding peaks between SPT6L and NRPE1. b Heatmaps of SPT6L, Pol II, and NRPE1 ChIP signals around peak center of all SPT6L peaks. The SPT6L peaks were clustered into two groups (genic and intergenic). The plotted regions are upstream and downstream 1 kb of peak center. c Binding profiles of SPT6L, Pol II, and NRPE1 at characterized six genomic groups. The six groups of regions were clustered according to the solo/double enrichment among the three proteins (see Supplementary Fig. 2d). The ChIP signal of each sample was averaged over two biological replicates. The plotted regions were 2 kb around the center of regions (upstream and downstream 1 kb, respectively). The y-axis value indicates the relative mean of normalized reads (1× sequencing depth normalization) per 10 bp non-overlapping bins. The number of regions in each group (G1 to G6) was indicated in the graph. d Heatmaps of the distance between enriched regions in each state and transcription start sites (TSS). Each rectangle represents 200 bp. e Co-immunoprecipitation (Co-IP) examined the interaction between SPT6L and core subunits of Pol V complex. Immunoprecipitation (IP) and Western blot (WB) were performed using specified antibodies (IP: anti-GFP, KTSM1301; WB: anti-Myc, ab9106, anti-GFP, Yeasen, 31002ES60). Data from two biological replicates were shown.