Fig. 4: The WG/GW repeat of SPT6L is dispensable for its intergenic enrichment and DNA methylation. | Nature Communications

Fig. 4: The WG/GW repeat of SPT6L is dispensable for its intergenic enrichment and DNA methylation.

From: A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis

Fig. 4

a The morphological phenotypes of 7-day old WT, spt6l, spt6l pSPT6L:SPT6L-GFP, and spt6l pSPT6L:SPT6LΔWG/GW seedlings. Bar = 0.5 mm. b Heatmaps of SPT6L and SPT6LΔWG/GW ChIP signals at SPT6L binding peaks. The plotted regions were similar to Fig. 1b and the strength of ChIP signals at 1 kb around of region centers were shown. c Scatterplot of SPT6L and SPT6LΔWG/GW ChIP signals in genomic binds. The Arabidopsis genome was divided into 100 bp length bins. ChIP signals (log2 values) in SPT6LΔWG/GW (y-axis) and SPT6L (x-axis) were plotted. d Genome tracks display the DNA methylation signals (in CG, CHG, and CHH contexts) of WT and spt6l SPT6LΔWG/GW on part of chromosome 5. Data from one biological replicate was shown. e Plots of DNA methylation levels in WT and spt6l SPT6LΔWG/GW within the genic and intergenic peaks of SPT6L. The plots displayed DNA methylation in CG, CHG, and CHH context. Data from one (spt6l SPT6LΔWG/GW) or two (WT) biological replicate(s) were shown.

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