Fig. 5: SPT5L is required for the intergenic enrichment of SPT6L.

a Genome tracks display the ChIP-seq signals of SPT6L, SPT6L spt5l, and NRPE1 on chromosome 1 (Chr1: 6700–6780 kb). Each sample was averaged over two biological replicates. b Binding profiles of SPT6L in WT and spt5l at four previously defined genomic groups. The ChIP signal of each sample was averaged over two biological replicates. The regions were plotted as indicated in Fig. 1c. c ChIP-qPCR showing genomic occupancy by NRPE1-GFP fusion protein in NRPE1-GFP and spt5l NRPE1-GFP. All the fold changes are relative to ChIP signal obtained at ACT7 in each sample and replicates. Error bars are presented as mean values ± s.d. from three biological replicates. All significant differences were indicated with *P < 0.05, **P < 0.01 (unpaired, two-tailed Student’s t-test). d Co-IP examined the interaction between SPT6L and SPT5L. Immunoprecipitation (IP) and Western blot (WB) were performed using specified antibodies (IP: anti-GFP, KTSM1301; WB: anti-Myc, ab9106, anti-GFP, Yeasen, 31002ES60). Data from two biological replicates were shown. e Co-IP examined the role of SPT5L in the interaction between SPT6L and NRPE1. IP and WB were performed using specified antibodies (same as Fig.5d). Data from two biological replicates were shown. f ChIP-qPCR showing genomic occupancy by SPT6L-GFP fusion protein in SPT6L-GFP, drm1 drm2 SPT6L-GFP, and nrpd1 SPT6L-GFP. All the fold changes are relative to ChIP signal obtained at ACT7 in each sample and replicates. Error bars are presented as mean values ± s.d. from three biological replicates. Different lowercase letters indicate significant differences, as determined by one-way ANOVA, P < 0.05.