Fig. 1: m6A-SAC-seq effectively identifies m6A sites across plant tissues. | Nature Communications

Fig. 1: m6A-SAC-seq effectively identifies m6A sites across plant tissues.

From: Quantitative profiling of m6A at single base resolution across the life cycle of rice and Arabidopsis

Fig. 1

a, b, Tissues from eight different rice organs (a) and nine different Arabidopsis organs (b), collected throughout their respective life cycles, were subjected to m6A-SAC-seq. c, d, mRNA m6A levels in the harvested samples were quantified using LC-MS/MS for both rice (c) and Arabidopsis (d). The m6A-to-A ratio was determined using calibration standards. Data are means ± SD, n = 6. e, Venn diagram showing m6A sites detected by SAC-seq overlapping with those identified by miCLIP and DRS, with the ±5 nt sliding window around each m6A site. f, A comparison of the percentages of overlapping m6A sites identified by different methods. g, h, The number of m6A sites and their motif distribution in rice (g) and Arabidopsis (h) tissues were shown. Motif sequences were separated into three groups, RAC, GAT and others. Base “R” denotes either A or G. 8D represents 8 days and 2 W represents 2 weeks. Source data are provided as a Source Data file.

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