Fig. 3: Three residues in the NB-ARC domain determine the functional difference between Pit1 and Pit2.

A Schematic architecture and cell death phenotype of Pit1 D485V, Pit2 D484V and domain-swapping mutants. Red and blue indicate domains derived from Pit1 and Pit2, respectively. The indicated proteins were transiently expressed in N. benthamiana, and cell death was photographed at 3 dpi. B Cell death phenotype of Pit1 D485V and the indicated amino acid substitution mutants in N. benthamiana, photographed at 3 dpi. Red and blue characters indicate residues derived from Pit1 and Pit2, respectively. C Infection assays of Pit1 mutant plants with the incompatible M. oryzae race 007.0. Photographs show phenotypes of representative Pit1 WT and Pit1 mutant plants at 7 dpi. Scale bar, 5 mm. D Biomass of the incompatible M. oryzae race was measured by qPCR and normalized with endogenous OsUbq. Relative infection ratio (Nipponbare (Nip.) = 1) is shown. Bars represent the mean ± s.d. (n  =  3 independent lines). Different letters above bars indicate a significant difference determined by one-way ANOVA (with Tukey’s test) (P < 0.05). E Cell death phenotype of Pit2 D484V and Pit2 mutants in N. benthamiana, photographed at 3 dpi. Red and blue characters indicate residues derived from Pit1 and Pit2, respectively. F Infection assays of Nipponbare expressing Pit2 mutants with the incompatible M. oryzae race 007.0. Photographs show phenotypes of representative Pit1 WT, Pit2 WT, and Pit2 LCG plants at 7 dpi. Scale bar, 5 mm. G Biomass of the incompatible M. oryzae race was measured by qPCR and normalized with endogenous OsUbq. Relative infection ratio (Nip. = 1) is shown. Bars represent the mean ± s.d. (n  =  3 independent lines). Different letters above bars indicate a significant difference determined by one-way ANOVA (with Tukey’s test) (P < 0.01). Source data are provided as a Source Data file.