Fig. 2: TE-derived siRNAs are differentially expressed in the sperm and vegetative cell lineages via Pol IV.
From: Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability
a Comparative small RNA analysis between transgenic and WT Col-0 pollen shows that siRNA depletion in pLAT52::RTL1 and pMGH3::RTL1 pollen occurs primarily at TEs. Among all TEs annotated in the Arabidopsis TAIR10 genome, the LTR/Gypsy superfamily was found over-represented in the lists of differentially expressed siRNA loci. b Different members of the LTR/Gypsy superfamily showed significantly reduced siRNA levels in pLAT52::RTL1 or pMGH3::RTL1 pollen. The bar plots show the fraction of TE superfamilies in the two datasets. c Many retrotransposons from the ATGP1 family lost siRNAs specifically in pLAT52::RTL1 pollen, while ATGP2 and ATGP2N lost siRNAs specifically in pMGH3::RTL1 pollen. The bar plots show the fraction of TE families in the two datasets. d siRNAs depleted in pLAT52::RTL1 or pMGH3::RTL1 pollen are dependent on Pol IV activity. Dots and bars represent expression level of individual replicates and mean values (n = 2), respectively, error bars represent the standard error, and RPM is reads per million. Source data are provided as a Source Data file.
