Fig. 2: Labeling and locations of FRET probes. | Nature Communications

Fig. 2: Labeling and locations of FRET probes.

From: Kinetics and mapping of Ca-driven calmodulin conformations on skeletal and cardiac muscle ryanodine receptors

Fig. 2

In silico predictions of the donor and acceptor fluorophore locations were conducted by simulated annealing for donors bound to FKBP, and by a method based on probability distributions for acceptors bound to CaM. Shown here are results for RyR2 (green) at low Ca2+ (EMDB: 9833, PDB: 6JI8)4. A The volume sampled by the fluorophore is shown as a pink bubble for the donor attached at FKBP residue 1. The average center position of the probe is shown as an orange sphere inside the pink bubble. The example CaM probe location is displayed as a pink bubble with the average acceptor location shown as a red sphere. B The predicted FRET distances from all donor-labeled sites on FKBP to acceptor-labeled site 26 in the N-lobe and site 99 in the C-lobe of CaM are shown with yellow and tan colored lines, respectively. The acceptor probe bound at CaM residue 110 is hidden behind the Handle domain.

Back to article page