Fig. 3: Representative FLT-FRET detection of distance distributions between D-FKBP and A-CaM probes. | Nature Communications

Fig. 3: Representative FLT-FRET detection of distance distributions between D-FKBP and A-CaM probes.

From: Kinetics and mapping of Ca-driven calmodulin conformations on skeletal and cardiac muscle ryanodine receptors

Fig. 3

At 30 nM Ca2+, SR membranes from skeletal muscle were labeled with D-FKBP (AF488-X-FKBP; X = Cys mutation site for fluorescence labeling), and then incubated with 800 nM A-CaM (labeled with acceptor probe at the N-lobe residue T26C). For each label site on FKBP, site 1 is maroon, site 6 is red, site 14 is orange, site 32 is yellow, site 44 is green, site 49 is teal, site 65 is blue and site 85 is purple. A Representative FLT-detected waveforms for each of the eight different D-FKBP. These waveforms were acquired at least three times with similar results. B Multi-exponential analysis of FLT-FRET data yielded a two-distance Gaussian distribution model for the separation between D-FKBP and A-CaM within RyR1. Averaged data shown in Supplementary Fig. 3. These waveforms were acquired at least three times with similar results. Source data are provided as a Source Data file.

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