Fig. 3: So(d18:1) inhibits HIF-2α transcription function in liver macrophages.
From: Sphingosine d18:1 promotes nonalcoholic steatohepatitis by inhibiting macrophage HIF-2α
A, B Flow cytometric and statistical analysis of chow-diet fed mice treated with control (5% CMC-Na), So(d16:1) and So(d18:1) (n = 5 per group). C GO:BP pathway enrichment showing the transcriptional level changes of some immune-related pathways. (n = 4 biologically independent samples). D Epas1 targets enrichment. E Relative mRNA levels of Hif2α and its downstream target genes in macrophages treated with vehicle, So(d18:1), So(d16:1), So(d20:1) or Sa(d18:1). (n = 3). F Representative immunoblot analysis of HIF-2α of BMDMs isolated from wild-type mice stimulated with the vehicle and So(d18:1) and BMDMs isolated from LysMHif2αLSL/LSL and Hif2α△LysM BMDMs stimulated with the vehicle. This experiment was repeated 3 times independently with similar results. G Representative immunoblot analysis of pro-caspase-1 and caspase-1 from Hif2α+/+ and LysMHif2αLSL/LSL BMDMs that were treated with So(d18:1) or not under NLRP3 inflammasome stimulation. This experiment was repeated 3 times independently with similar results. H, I Protein level of IL-1β (H), IL-18 (I) from Hif2α+/+ and LysMHif2αLSL/LSL BMDMs treated with So(d18:1) or not under NLRP3 inflammasome stimulation. (n = 6 biologically independent samples). Data are the means ± s.e.m. B, E, H, I Statistical analysis was performed using One-Way ANOVA test; Spint in (E), statistical analysis was performed using the Kruskal–Wallis test. C One-sided hypergeometric distribution test, using Benjamin Hochberg method for p-value correction. D One-sided permutation test without p-value correction.
