Fig. 4: HIF-2α KO in macrophages accelerated inflammation and fibrosis in NASH mice.
From: Sphingosine d18:1 promotes nonalcoholic steatohepatitis by inhibiting macrophage HIF-2α
Eight-week-old male Hif2αfl/fl and Hif2αΔLysm mice were administered a GAN diet for 24 weeks (SPF, n = 6 mice/group). A Liver weights. B Ratios of liver mass to body mass. C Serum ALT. D Serum AST. E Representative H&E (up), and Sirius Red (down) staining of liver sections. The circles marked the inflammation foci. n = 3 mice per group, 3 images per mouse. Scale bar is 200 μm. F The percentage of fibrosis area. G–J Histology scores of hepatic steatosis (G), lobular inflammation (H), ballooning (I), and NAFLD activity (J). K, L Relative mRNA levels of genes related to hepatic inflammation (K) and fibrosis (L). n = 6 biologically independent samples. Data are the means ± s.e.m. A–D, F, K, L Statistical analysis was performed using two-tailed Student’s t-tests; G–J Col2a1 in (L), statistical analysis was performed using two-tailed Mann–Whitney U-tests.
