Fig. 5: HIF-2α overexpression in macrophages ameliorated inflammation and fibrosis in NASH mice.
From: Sphingosine d18:1 promotes nonalcoholic steatohepatitis by inhibiting macrophage HIF-2α
Eight-week-old male Hif2α+/+ and LysMHif2αLSL/LSL mice treated with or without So(d18:1) by daily intraperitoneal injection under CDAA-HFD for 8 weeks (SPF, n = 6 mice/group). A Liver weights. B Ratios of liver mass to body mass. C Serum ALT. D Serum AST. E Representative H&E (up), and Sirius Red (down) staining of liver sections. The circles marked the inflammation foci. n = 3 mice per group, 3 images per mouse. Scale bar is 100 μm. F The percentage of fibrosis area. G–J Histology scores of hepatic steatosis (G), lobular inflammation (H), ballooning (I), and NAFLD activity (J). K Relative mRNA levels of genes related to hepatic inflammation. L Relative mRNA levels of genes related to hepatic fibrosis. n = 6 biologically independent samples. Data are the means ± s.e.m. A–D, F, K, L Statistical analysis was performed using one-way ANOVA; G–J Ccl2 in (K), Timp1, Col3a1, Col4a2 and Col5a2 in (L), statistical analysis was performed using the Kruskal–Wallis.
