Fig. 7: FG-4592 significantly mitigates CDAA-HFD diet-induced NASH.
From: Sphingosine d18:1 promotes nonalcoholic steatohepatitis by inhibiting macrophage HIF-2α
CDAA-HFD-fed mice were treated with vehicle or FG-4592 for 8 weeks (n = 6 mice/group). A Liver weights. B Ratios of liver mass to body mass. C Serum ALT. D Serum AST. E Representative H&E (up), and Sirius Red (down) staining of liver sections. The circles marked the inflammation foci. n = 3 mice per group, 3 images per mouse. Scale bar is 100 μm. F The percentage of fibrosis area. G–J Histology scores of hepatic steatosis (G), lobular inflammation (H), ballooning (I), and NAFLD activity (J). K, L Relative mRNA levels of genes related to hepatic inflammation (K) and fibrosis (L). n = 6 biologically independent samples. Data are the means ± s.e.m. A–D, F, K, L Statistical analysis was performed using two-tailed Student’s t-tests; G–J Il1b, Il6 in (K), Timp1 and Col5a2 in (L), statistical analysis was performed using two-tailed Mann–Whitney U-tests.
