Fig. 6: Conditional loss of Ankrd11 in the neural crest causes failure of asymmetric TGF-β signaling in OFT cushions at E11.5 that is partially restored by E12.5. Related to Supplemental Fig. 4. | Nature Communications

Fig. 6: Conditional loss of Ankrd11 in the neural crest causes failure of asymmetric TGF-β signaling in OFT cushions at E11.5 that is partially restored by E12.5. Related to Supplemental Fig. 4.

From: The chromatin regulator Ankrd11 controls cardiac neural crest cell-mediated outflow tract remodeling and heart function

Fig. 6

a–p Representative images of E11.5 Ankrd11nchet (a, b, e, f, i, j, m, n) and Ankrd11ncko (c, d, g, h, k, l, o, p) distal and medial OFT, in the parietal (PC) and septal (SC) cushions (outlined with white dashed lines) at E11.5 (a–h) and E12.5 (i–p) immunostained for CNCCs (YFP; green), pSmad2/3 (white) and counterstained for nuclei (Hoechst, magenta). q–t Quantification of a–p for percent of pSmad2/3 CNCCs (% pSmad2/3 + YFP + /YFP+ cells) in Ankrd11nchet (orange) and Ankrd11ncko (green) medial OFT (q, s) and in the Ankrd11ncko distal OFT (r, t), at E11.5 (q, r) and E12.5 (s, t), in the parietal and septal cushions. Two-tailed multiple t-tests with Holm-Sidak multiple comparisons test, medial (q, Ankrd11nchet p = 0.030585; s, Ankrd11nchet p = 0.004133, Ankrd11ncko p = 0.002271); two-tailed unpaired t-test, distal. Graphs represent mean ± s.e.m; n = 3 Ankrd11nchet and n = 3 Ankrd11ncko samples from at least 2 independent litters. ns: not significant, *p < 0.05, **p < 0.01. Scale bars: 100 µm. Source data are provided as a Source Data file.

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