Fig. 7: MERFISH identified CNCC clusters in the OFT at E11.5. Related to Supplemental Fig. 5. | Nature Communications

Fig. 7: MERFISH identified CNCC clusters in the OFT at E11.5. Related to Supplemental Fig. 5.

From: The chromatin regulator Ankrd11 controls cardiac neural crest cell-mediated outflow tract remodeling and heart function

Fig. 7

a Plot showing the spatial position of cells from representative transverse sections of E11.5 Ankrd11nchet (left) and Ankrd11ncko (right) embryos subjected to MERFISH with a custom 140 gene panel (Supplementary Data 1). A red bounding box defining the OFT is magnified and shown with cell clusters in the center. Cells in the OFT are colored according to Leiden clustering (clusters 1-13). b, c Plots showing the position of OFT cells from all samples in UMAP-space. Cells are colored according to Leiden clustering in b and according to genotype in c. d Heatmap plot showing scaled expression of Penk, Pdgfra, Sox9, and Postn in OFT cells from all samples. Cells are ordered according to their clustering. e Bar plot showing the proportion of all CNC-classified cells belonging to different genotypes and clusters (Ankrd11nchet: cluster 2, 518 cells; cluster 3, 306 cells; cluster 7, 152 cells. Ankrd11ncko: cluster 2, 475 cells; cluster 3, 632 cells; cluster 7, 249 cells). f Plots showing spatial positions of OFT cells colored according to normalized gene expression. Left column shows cells from representative Ankrd11nchet sample, right column shows cells from representative Ankrd11ncko sample. n = 3 Ankrd11nchet and n = 3 Ankrd11ncko samples from at least 2 independent litters. Source data are provided as a Source Data file.

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