Fig. 2: Compensatory changes in gene expression, embryo length, cuticle, and viability.
From: Rapid response of fly populations to gene dosage across development and generations
a–d Eve stripes in Population 2-6-1 A (anti-Eve staining), with the arrow in (d) showing a prominent anterior shift in the 7th stripe. The shifts were quantified in (c) from in situ data (eve co-stained with sna). N = 21, 12, 13, and 16 for WT, Gen. 4, Gen. 8 and Gen. 10 respectively. e Embryo length. N = 21, 33, 13, and 41 for WT, Gen. 4, Gen. 8 and Gen. 10 respectively. f–i tailless (tll) expression, detected by in situ hybridization. i shows the normalized intensity profiles aligned at the posterior end. Solid lines are average tll intensity and the shaded panels denote the standard deviation. N = 22 and 14 for Gen. 4 and Gen. 8, respectively. j The number of nuclei from the posterior boundary of eve stripe 7 to the posterior pole. N = 12 and 11 for Gen. 4 and Gen. 8, respectively. k Rescue of cuticle defects. N = 41, 34, and 32 larvae. l Viability to adulthood, presented as mean values +/- standard error. Viability was measured from ~100 embryos per replicate, with 1–3 replicates per group (also see Fig. S1). Scale bar = 100 um. G0 in (k) and (l) represents a non-mutagenized 4xbcd stock. All boxplots in this work are defined as follows: center line, median; box limits, the first and third quartiles; whiskers, 1.5x interquartile range. Source data are provided as a Source Data file.
