Fig. 2: Sema reverses cardiac hypertrophy, fibrosis and dysfunction in mice that underwent TAC for eight weeks. | Nature Communications

Fig. 2: Sema reverses cardiac hypertrophy, fibrosis and dysfunction in mice that underwent TAC for eight weeks.

From: Semaglutide ameliorates cardiac remodeling in male mice by optimizing energy substrate utilization through the Creb5/NR4a1 axis

Fig. 2

A The time of TAC and Sema treatment (i.p.) in mice. B Representative B- and M-mode echocardiographic imaging of the heart. C, D Echocardiographic analysis of EF and FS in mice, data are presented as mean ± SEM (n = 6); EF: F (6, 88) = 25.78, P = 1.8e-017, F (1.947, 85.69) = 75.90 P = 2.0e-019, F (3, 44) = 124.5, P = 1.6e-021, F (44, 88) = 0.8118, P = 7.8e-001, F (6, 132) = 27.50, P = 4.1e-021, F (2, 132) = 80.98, P = 1.1e-023, F (3, 132) = 107.9, P = 2.4e-035; FS: F (6, 132) = 58.51, P = 7.9e-035, F (2, 132) = 144.4, P = 5.9e-034, F (3, 132) = 192.9, P = 4.6e-048, F (6, 132) = 58.51, P = 7.9e-035, F (2, 132) = 144.4, P = 5.9e-034, F (3, 132) = 192.9, P = 4.6e-048. E Representative histopathological cross-sectional images of mice hearts (scale bar: 1 mm; n = 6). F, G Myocardial hypertrophy was detected by WGA staining and then quantitatively analyzed (scale bar: 50 μm; n = 6); CSA: F (3, 20) = 70.58, P = 8.1e-011. H, I Myocardial fibrosis was detected by PSR staining and quantification of the collagen volume (scale bar: 50 μm; n = 6); Fibrotic area: F (3, 20) = 225.5, P = 1.4e-015. J The ratio of BW, HW, and TL (n = 12); HW/BW: F (3, 44) = 180.2, P = 1.0e-024; HW/TL: F (3, 44) = 140.2, P = 1.5e-022. K The mRNA expression levels of ANP, BNP, collagen I and collagen III in the heart (n = 6); ANP and BNP: F (7, 40) = 138.8, P = 5.2e-026; COLI and COL III: F (7, 40) = 82.22, P = 1.0e-021. All results are shown as the mean ± SEM and analyzed using one-way ANOVA followed by Bonferroni post hoc test G, I and J, K. For the analysis in (C,D), repeated measures two-way ANOVA followed by Sidak post hoc test was conducted. p values are indicated. Source data are provided as a Source Data file. EF, ejection fractions; FS, fractional shortening; LW, lung weight; HW, heart weight; TL, tibial length; BW, body weight; BNP, B-type natriuretic peptide; ANP, A-type natriuretic peptide; WGA, wheat germ agglutinin; PSR, picrosirius red.

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