Fig. 6: NR4a1 knockdown ameliorates disorders of glucolipid metabolism and ameliorates pathological cardiac remodeling. | Nature Communications

Fig. 6: NR4a1 knockdown ameliorates disorders of glucolipid metabolism and ameliorates pathological cardiac remodeling.

From: Semaglutide ameliorates cardiac remodeling in male mice by optimizing energy substrate utilization through the Creb5/NR4a1 axis

Fig. 6

A Representative B- and M-mode echocardiographic imaging of shRNA/shNR4a1 mouse hearts. B Echocardiographic analysis of EF and ES in mice (n = 12); EF: F (4, 55) = 48.91, P = 1.7e-017; FS: F (4, 55) = 130.9, P = 2.0e-027. C, D Myocardial hypertrophy was detected by WGA staining and then quantitatively analyzed in shRNA/shNR4a1 mice (n = 6); F (3, 396) = 2018, P = 1.70e-239. E, F Western blot analysis and quantification of the mitochondrial respiratory chain proteins ATP5a, UQCRC2, MTCO1, SDHB and NDUF88 in the cardiac tissue of shRNA/shNR4a1 mice after sham or TAC surgery (n = 6 independent experiments with similar results); F (12, 100) = 1.789, P = 6.0e-002, F (4, 100) = 161.5, P = 1.0e-042, F (3, 100) = 62.10, P = 9.4e-023. G Mass spectrometry-based analysis of glycolipid metabolism and TCA-related substances in shRNA/shNR4a1 mice 8 weeks after sham or TAC surgery (scale bar: 50 μm; n = 6). All results are shown as the mean ± SEM, and analysis using one-way ANOVA followed by Bonferroni post hoc test (B, D and F) was conducted. p values are indicated. Source data are provided as a Source Data file. EF, ejection fractions; FS, fractional shortening; WGA, wheat germ agglutinin; TAC, transverse aortic constriction.

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