Fig. 4: vgRNA clusters are encapsulated in membranes of remodeled ER.
From: Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles
a Representative confocal images of SARS-CoV-2 infected cells indicate an appearance of dense perinuclear foci of Sec61β ER labeling (green) at 24 hpi that colocalizes with vgRNA and nsp3. b SR images reveal concentric organization of Sec61β around vgRNA, dsRNA and nsp12, and colocalization of Sec61β with nsp3. c Bivariate pair-correlation functions indicate anti-correlation of Sec61β with vgRNA and dsRNA and association of Sec61β with nsp3. Scale bars, 20 µm (a) and 1 µm (b). Dashed lines in b indicate the boundary of the cell nucleus.
