Fig. 5: Nsp3 localizes at the surface of vgRNA clusters.
From: Nanoscale cellular organization of viral RNA and proteins in SARS-CoV-2 replication organelles
a Representative confocal images of a SARS-CoV-2 infected cell display DL colocalization between punctate vgRNA (magenta) and nsp3 (green) labeling at 6 hpi. b Representative SR image of a SARS-CoV-2 infected cell at 6 hpi. c Zoomed-in images of selected vgRNA particles (yellow boxes in b) indicate the localization of nsp3 at the surface of the vgRNA clusters. d Magnified region with aggregates of vgRNA clusters (blue box in b) displays dense nsp3 localization in the core of these aggregates. e Bivariate pair-correlation functions calculated between the SM localizations of vgRNA and nsp3 indicate nanoscale anti-correlation of these targets at 6 hpi. f Confocal images show that vgRNA and nsp3 occupy approximately the same regions in a SARS-CoV-2 infected cell at 24 hpi. g Representative SR image of a SARS-CoV-2 infected cell at 24 hpi. h, i Magnified regions of the SR image (colored boxes in g) reveal that nsp3 localizes in interstitial regions or encapsulates vgRNA clusters. j Bivariate pair-correlation functions indicate nanoscale anti-correlation between vgRNA and nsp3 at 24hpi. Scale bars, 10 µm (a, f), 2 µm (b, g), 500 nm (d, h, i), 200 nm (c). Dashed lines in b and g indicate the boundary of the cell nucleus.
