Fig. 1: The chromosome-scale reference-genome of Bufo(tes) viridis, its karyotype and comparison with the reference genome of Bufo bufo.

a Breeding pair of B. viridis in amplexus (© M. Stöck). b Giemsa-stained karyotype of B. viridis (© M. Stöck). c B. viridis-assembly (y-axis) compared to B. bufo (x-axis) assembly by the Vertebrate Genome Project (VGP, GenBank accession number GCA_905171765.1) showing high synteny of both bufonid genomes across all 11 chromosomes.