Fig. 3: Additive sex-specific XX- or XY-expression of bod1l and expression of the ncRNA-Y in females and males in seven developmental stages, with numbers (N) of females and males (N) per stage shown in bars, genetically sexed using PCR-marker BvXY1.

Gosner⁴³ stages 23–24, 10 days after fertilization, Gosner stages 27–29: 15 days after fertilization, Gosner stages 30–33: 18 days after fertilization, Gosner stages 34–38: 34 days after fertilization, Gosner stages 43–44: beginning of metamorphosis, Gosner stages 45–46, including toads up to six weeks after metamorphosis, the latter with anatomically visible differentiation of testes and Bidder’s organs in males, and ovaries in females, subadult male and adult female. Numbers of available tadpole transcriptomes varied by stage; TPM: Transcript reads Per Million RNAseq reads; note that TPM for the ncRNA-Y were multiplied 20x. See Supplementary Fig. 8 for comparison of additive sex-specific expression of bod1l and ncRNA-Y and haplotype-specific bod1l-expression in males. Bars present mean values; error bars show the standard deviation of the respective data points.