Fig. 1: Binding of mBcs1L to ISP-ED and elevated ATPase activity. | Nature Communications

Fig. 1: Binding of mBcs1L to ISP-ED and elevated ATPase activity.

From: Conformations of Bcs1L undergoing ATP hydrolysis suggest a concerted translocation mechanism for folded iron-sulfur protein substrate

Fig. 1

A mBcs1L ATPase activities in the presence of ISP-ED at different ISP:mBcs1L heptamer ratios. Data shown are mean ± SD of three independent experiments (n =3). Source data are provided in Supplementary Table 1. B Western blot showing pulldown of ISP-ED by Ni-NTA resin only in the presence of His-tagged mBcs1L. ISP-ED was mixed with Ni-NTA resin in the presence or absence of histidine-tagged mBcs1L. Samples were taken following wash and elution steps and were analyzed by SDS-PAGE and Western analyses. Uncropped scans of blots are provided in the Source Data file.

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