Fig. 2: Multiple clusters of polyfunctional effector memory CD4+ T cells increase after vaccination. | Nature Communications

Fig. 2: Multiple clusters of polyfunctional effector memory CD4+ T cells increase after vaccination.

From: Adolescent BCG revaccination induces a phenotypic shift in CD4+ T cell responses to Mycobacterium tuberculosis

Fig. 2

a Based on flow cytometry data, heatmap showing the proportion of cells within the 20 PhenoGraph clusters expressing each marker. Each column represents a unique cluster in one or both vaccine groups. The percentage of cells in a given cluster expressing each of the markers evaluated is shown numerically; markers expressed in fewer than 20% of the cells are not annotated. Graph above the heatmap shows the median number of cells per participant in each cluster by vaccine arm with error bars extending to no further than 1.5 times the inter-quartile range. b Heatmap showing fold-change in the frequency of CD4+ T cells in each of the clusters that showed a significant change over pre-vaccination (Day 0) in at least one timepoint in at least one of the vaccine groups. *FDR q < 0.05; **FDR q < 0.01. c Percentage of total activated CD4+ T cells for each significant cluster over the time course. Ag85B (light blue) and TB10.4 (dark blue) peptide pool stimulations; for the BCG group (n = 22 biologically independent samples), only the BCG (light red), M.tb lysate (dark red) stimulations. *FDR q < 0.05, two sided Wilcoxon Signed Rank Test. Boxplots indicate the median response and the first and third quartiles; whiskers extend to no further than 1.5 times the inter-quartile range. Source data are provided as a Source Data file.

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