Fig. 5: TB vaccination boosts multiple TEM clusters.
a Proportion of activated T cells of total CD4+ T cells, measured by CITE-Seq, present in each TEM cluster that showed a significant change after vaccination in either the BCG (n = 15) or the H4:IC31 (n = 8) vaccine groups. *p < 0.05, **<0.01, two sided Wilcoxon Signed Rank test. Each point depicts the response in a single participant. Boxplots indicate the median response and the first and third quartiles; whiskers extend to no further than 1.5 times the inter-quartile range. b Ridge plot showing normalized expression values of antibody-derived tags (ADT) for selected surface markers for the TEM CD4+ T cell clusters. c Heatmap showing average gene expression by participant (columns) of the top 20 genes (rows) that distinguish the 6 antigen-reactive TEM CD4+ T cell clusters from each other. d Overlay showing cells expressing transcripts of selected cytokines on the UMAP projection of all activated CD4+ T cells. Cluster numbers are shown on the figure.
