Fig. 1: Experimental design to study bacterial response to TET. | Nature Communications

Fig. 1: Experimental design to study bacterial response to TET.

From: Bactericidal effect of tetracycline in E. coli strain ED1a may be associated with ribosome dysfunction

Fig. 1

Integrative approach to investigate the short and prolonged response to TET of the lab strain E. coli K-12 and the gut isolate E. coli ED1a. Both strains were exposed to TET for 30 min and 180 min. Upper panel: a schematic viability diagram (upper right) displaying the differences in survival between two E. coli strains. The black line illustrates the bacteriostatic effect of TET on K-12, the red line – bactericidal effect on ED1a. The gray line illustrates the growth of untreated cells. White dots represent the timepoints used in the study. Lower panel: the structure of the respective TET binding site on the 70S ribosomes was addressed by single particle cryo-EM; changes in proteome abundance and thermal stability by thermal proteome profiling, cell morphology by in situ cryo-ET, and the translation states of ribosomes by subtomogram averaging.

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