Fig. 5: FBXO7 inhibits serine synthesis and promotes oxidative stress by downregulating PRMT1 in HCC cells.
From: FBXO7 ubiquitinates PRMT1 to suppress serine synthesis and tumor growth in hepatocellular carcinoma
a Schematic of U-[13C]-glucose incorporation into serine and glycine in cells. b, c Incorporation of U-[13C]-glucose carbon into serine (b) and glycine (c) detected by LC–MS/MS in cells overexpressing FLAG-FBXO7 and GFP-PRMT1-WT or K37R. Data are presented as the mean ± SD (n = 3 independent experiments). Statistical analysis was performed using the two-tailed Student’s t-test. d, e GSH (d) and ROS (e) levels in FBXO7 and/or PRMT1 KD cells cultured in CM or -SG medium. Data are presented as the mean ± SD (n = 3 independent experiments). Statistical analysis was performed using the two-tailed Student’s t-test. f ROS levels in cells overexpressing FLAG-FBXO7 and GFP-PRMT1-WT or K37R cultured in CM or -SG medium. Data are presented as the mean ± SD (n = 3 independent experiments). Statistical analysis was performed using the two-tailed Student’s t-test. g ROS levels in FLAG-FBXO7-overexpressing cells cultured in -SG medium in the presence or absence of NAC (5 mM). Data are presented as the mean ± SD (n = 3 independent experiments). Statistical analysis was performed using the two-tailed Student’s t-test. Source data are provided as a Source Data file.
