Fig. 6: FBXO7 suppresses HCC growth by downregulating PRMT1.

a Growth rates of FBXO7 and/or PRMT1 KD cells grown in CM or −SG medium. Data are presented as the mean ± SD (n  =  5 independent experiments). Statistical analysis was performed using the two-way ANOVA with Bonferroni correction. b Growth rates of cells overexpressing FLAG-FBXO7 and GFP-PRMT1-WT or K37R grown in CM or −SG medium. Data are presented as the mean ± SD (n  =  5 independent experiments). Statistical analysis was performed using the two-way ANOVA with Bonferroni correction. c Growth rates of FLAG-FBXO7-overexpressing cells grown in -SG medium in the presence or absence of NAC (5 mM). Data are presented as the mean ± SD (n  =  5 independent experiments). Statistical analysis was performed using the two-way ANOVA with Bonferroni correction. d Immunoblotting analysis of cleaved caspase 3 and caspase 3 in FLAG-FBXO7-overexpressing cells grown in −SG medium in the presence or absence of NAC (5 mM). The immunoblotting experiments were repeated three times with similar results. e, f FBXO7 and/or PRMT1 KD Huh7 cells were subcutaneously inoculated into nude mice fed with a +SG or −SG diet. Tumor image (e) and weight (f) were shown. Data are presented as the mean ± SD (n = 6 mice). Statistical analysis in f was performed using the two-tailed Student’s t-test. g, h Huh7 cells overexpressing FLAG-FBXO7 and GFP-PRMT1-WT or K37R were subcutaneously inoculated into nude mice fed with a +SG or −SG diet. Tumor image (g) and weight (h) were shown. Data are presented as the mean ± SD (n = 5 mice). Statistical analysis in h was performed using the two-tailed Student’s t-test. i Representative images of IHC staining for Ki-67, 8-oxo-dG, and cleaved caspase 3 in tumor xenografts in (g). Scale bars, 50 μm. Source data are provided as a Source Data file.