Fig. 1: Gene expression variation between TG1-WT and TG2-WT upon exposure to high light intensity.

RNA-seq data collected from wild type P. celeri cultures after 1 h of exposure to HL conditions following acclimation to LL (T = 0). Changes in transcript abundances of (A) TG1 and (B) TG2 cultures expressed as Log2 of the fold changes between 1 h high light and steady state low light growth. Dashed lines represent threshold values for fold change and p-values (L2FC(1) and 0.05, respectively) used to identify significant gene sets, as determined by a two-sided Wald test using Benjamini–Hochberg multiple testing adjustment. The putative CCA1 transcript is labeled in each with its transcript ID number, and not significant (NS) genes colored in gray. C Comparison of significant differentially expressed gene sets between TG1 and TG2. The sets identified in above panels were separated into up or down regulated groups and all combinations of comparison are represented in the lower matrix with the number of members shown in the above bars. D Statistical analysis to deconvolute P. celeri TG1 and TG2 isolates’ transcriptome. The results of a two-sided likelihood ratio test determining the significance of each gene to a model isolating the interaction term between high light exposure time and strain is displayed as color scaled p-values over the fold change observed for each gene. All data shown are derived from independent biological replicate cultures (n = 3). Source data are provided as a Source Data file.