Fig. 2: Transcriptional responses of Picochlorum celeri TG1 and TG2 isolates and engineered TG1-MYB99 strain, in response to high intensity light shift.

A The relative position of each sample is displayed based on a principal component analysis scores plot of the regularized log transform data of the 500 most highly expressed transcripts. B CCA1 transcriptional dynamics over the acclimation time course. Relative transcript levels were computed following alignment to a reference with a single CCA1, and therefore represent the total pool of TG1 and TG2 transcript isoforms. C Time course transcript dynamics of genes clustered based on their similarity to CCA1 expression are displayed by their deviation from median transcript levels of the cluster across the time course with boxplots representing distributions of expression level variances for genes averaged from independent biological replicate cultures (n = 3) centered on the median with lower and upper bounds on the 25th and 75th percentiles, respectively. Lower and upper whiskers indicate minimum and maximum values up to 1.5 times interquartile range in each direction. The corresponding bar plot (D) of enriched functional annotation categories indicates the number of genes with each annotation type and colored by the significance of their over-representation compared to the remaining background genome as computed using a hypergeometric distribution with Benjamini–Hochberg multiple testing adjustment.